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CCR Molecular Pathology Unit (MPU)

Special Equipment / Training Available within The Molecular
Pathology Unit

Laboratory of Cancer Biology and Genetics - NIH, Bethesda, MD Bldg. 37, room 2002

  • Procedure room, downdraft table
  • Digital slide conferencing
  • Digital slide scanner / digital image analysis
  • Laser microdissection instrument
  • Bioluminescent optical imager
  • Molecular Biology Workshop (LINK)
  • Library (Link)
  • Photomicroscopy
  • Multispectral image analysis
  • Video-teleconferencing
  • Telepathology
  • Light and fluorescence microscopy
  • Stereomicroscopy

Molecular Pathology Workshop

current traineesMolecular Pathology Workshop is a laboratory based course for fellows arriving at the NIH. This multi-week workshop addresses common experiments and theories in molecular biology. The course can be adjusted to the needs and level of experience of each trainee. During their transition from didactic graduate course work and clinically oriented backgrounds in veterinary medicine and pathology, trainees receive hands-on laboratory bench experience applicable to molecular diagnostics and their dissertation research.

Experiments may include:

  • Procurement of Tissues for Molecular Analysis
  • Genomic DNA Isolation from Tissue
  • Spectrophotometry
  • Polymerase Chain Reaction
  • Agarose Gel Electrophoresis
  • DNA Isolation from Agarose Gels
  • Subcloning
  • Plasmid DNA Isolation
  • Restriction Enzyme Digestion
  • RNA Isolation from Tissues
  • cDNA Synthesis
  • Real Time PCR
  • Cell Culture
  • Cytospin Preparation
  • Protein Lysate Preparation
  • BCA Protein Assay
  • SDS-PAGE Gel Electrophoresis
  • Coomassie Blue Protein Gel Staining
  • Western Blotting
  • Immunohistochemistry
  • Digital Slide Scanning and Image Analysis
  • Laser Capture Microdissection

Back to Top • This page last reviewed: April 15, 2014


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